Additional file 1: of Concentrations of persistent organic pollutants in maternal and cord blood from the maternal-infant research on environmental chemicals (MIREC) cohort study

Results of Statistical Hypothesis Testing—MIREC Persistent Organic Pollutants Analysis-Using censoring methods. Table S1 Results for MIREC persistent organic pollutants in maternal blood by Parity (μg/L). Table S2 Results for MIREC persistent organic pollutants in maternal blood by maternal age (μg/L). Table S3 Results for MIREC persistent organic pollutants in maternal blood by smoking status (μg/L). Table S4 Results for MIREC persistent organic pollutants in maternal blood by household income (μg/L). Table S5 Results for MIREC persistent organic pollutants in maternal blood by pre-BMI (μg/L). Table S6 Results for MIREC persistent organic pollutants in maternal blood by place of birth (μg/L). Table S7 Results for MIREC persistent organic pollutants in maternal blood by fasting (μg/L). Table S8 Results for MIREC persistent organic pollutants in maternal blood by maternal education (μg/L). Table S9 Results for MIREC persistent organic pollutants in maternal blood by whether using non-stick cooking vessels. Table S10 Results for MIREC persistent organic pollutants in maternal blood by whether using non-stick cooking vessels in the microwave. Table S11 Results for MIREC persistent organic pollutants in maternal blood by year of collection. Table S12 Results for MIREC persistent organic pollutants in maternal blood by intake of bacon. Table S13 Results for MIREC persistent organic pollutants in maternal blood by intake of fish. Table S14 Results for MIREC persistent organic pollutants in maternal blood by intake of Hamburger. Table S15 Results for MIREC persistent organic pollutants in maternal blood by intake of pork. Table S16 Results for MIREC persistent organic pollutants in maternal blood by intake of poultry. Table S17 Results for MIREC persistent organic pollutants in maternal blood by intake of steak. Table S18 Comparison of demographic groups when the INTERACTION between BMI and total lipid was significant. Table S19 Comparison of demographic groups when the INTERACTION was significant between year of collection and total lipid. Table S20 Comparison of demographic groups when the INTERACTION was significant between intake of bacon and total lipid. Table S21 Results for MIREC persistent organic pollutants in cord blood by infant gender (μg/L). Table S22 Results for MIREC persistent organic pollutants in cord blood by season of collection (μg/L). Table S23 Results for MIREC persistent organic pollutants in cord blood by smoking status of mother (µg/L). (DOCX 207 kb

Schematic illustration of the experimental design.

<p>A) Human endometrial tissue was inoculated into the peritoneal cavity of mice using a micropipette and left for 12 days before starting treatment. ISO-1 was then intra-peritoneally administered on a daily basis for 14 consecutive days before euthanizing the animals. B) Size of injected endometrial fragments.</p

Real time PCR analysis of the expression of MMP2, MMP9, TIMP1, VEGF, COX2 and IL8 in endometriotic lesions.

<p>Lesions harvested from mice treated with vehicle (control) or 4 mg/kg ISO-1. mRNA levels were normalized to that of the house-keeping gene GAPDH. Results were from 8 control mice and 7 mice treated with ISO-1. Data are mean ± SEM. *, **, ***, P<0.05, P<0.01 and P<0.001, respectively, as compared to the control group.</p

Real time PCR analysis of the expression of Bax, Bcl2, integrin αv and integrin β3 in endometriotic lesions.

<p>Lesions were harvested from mice treated with vehicle (control) or 4 mg/kg ISO-1. mRNA levels were normalized to that of the house-keeping gene GAPDH. Results were from 8 control mice treated with vehicle and 7 mice treated with ISO-1. Data are mean ± SEM. *, **, P<0.05 and P<0.01, respectively, as compared to the control group.</p

Histological examination of human endometrial implants in nude mice two weeks after inoculation of endometrial tissue.

<p>A) Initial endometrial tissue before inoculation into mice. B) Endometrial implant 12 days following tissue inoculation showing epithelial glands (EG) surrounded by endometrial stromal cells (S) and mouse tissue (MT). Hematoxylin-eosin staining; scale bar, 20 µm. C and D) Effect of endometrial tissue inoculation and treatment with ISO-1 on the survival rate of animals. E and F) Effect of endometrial tissue inoculation and treatment with ISO-1 on the body weight of animals. The Figure summarizes results obtained from 7 mice treated with ISO-1 (4 mg/kg) and 8 control mice treated with vehicle.</p

List of PCR primers.

<p>List of PCR primers.</p